[kw] ウシ・ウイルス試験受託機関

VMRD

Virus Bank Characterization

9CFR VIRUS TESTING

These assays evaluate the test articles for extraneous viruses as specified in USDA-APHIS 9 CFR 113.52, 113.53c, 113.55, including compliance with 113.46 and 113.47. VMRD tests for viruses in materials from all mammalian species listed in UDSA-APHIS 9 CFR 113.47.

Bovine Polyomavirus (BPyV) Testing

VMRD’s validated 21 day assay for the detection of infectious BPyV is based on growth in bovine cells followed by an IFA detection of infective BPyV using a mono-specific antiserum generated against a BPyV subunit protein.This assay can reliably detect as little as 10-7 MOI (0.2 virions) in test articles.

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Other その他

検索結果 (関連文献)

Detection of infectious Bovine polyomavirus | Request PDF (researchgate.net)

ResearcGateで検索された文献一覧．

Virome Characterization in Commercial Bovine Serum Batches—A Potentially Needed Testing Strategy for Biological Products (2021)

商用製品には，Fetal Bovine Serum (FBS), Newborn Calf Serum (NCS), Bovine Calf Serum (BCS), and Donor Bovine Serum (DBS)があり，今回使用したサンプルは，DBSを除く血清である．

BPyVにはγ線照射は余り効果的ではなく(1~2ログ)，粒子径が小さい(45nm)であるため0.2μmろ過による除去は期待できない．